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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: An imbalanced ratio between PC(16:0/16:0) and LPC(16:0) revealed by lipidomics supports the role of the Lands cycle in ischemic brain injury
doi: 10.1074/jbc.RA120.016565
Figure Lengend Snippet: LPC(16:0) causes growth cone collapse and neuronal death. A , eGFP transgenic mouse brain cortical explants were cultured for 3 days and treated with the agent as indicated on the microphotograph. Growth cones are indicated by yellow-colored arrows, and collapsed growth cones are shown by the red-colored arrows. The scale bar = 10 μm. B , the % of collapsed growth cones was counted and plotted (n = 10 independent explants per treatment). Neurite length of cortical explants treated with 10 μM of the indicated agent was measured using the Scholl method and plotted in C . The neurite lengths of cortical explants treated with LPC(16:0) ( D ) and PC(16:0/16:0) ( E ) at different doses were plotted. Cortical neuronal cultures treated with the indicated LPC(16:0) ( F ) and PC(16:0/16:0) ( G ) were examined for cell viability using the CCK-8 kit. DMSO and glutamate treatment were used as negative and positive controls, respectively, for neuronal death. Data represent the mean ± SD. Error bars indicate SD. n = 5 independent repeats, ∗ indicates p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by one-way ANOVA with LSD post hoc analysis to identify significant groups.
Article Snippet: The
Techniques: Transgenic Assay, Cell Culture, CCK-8 Assay